Journal: Cancer Cell International

Article Title: HOPX is a tumor-suppressive biomarker that corresponds to T cell infiltration in skin cutaneous melanoma

doi: 10.1186/s12935-023-02962-2

Figure Lengend Snippet: HOPX inhibits proliferation, migration, and invasion and induces apoptosis and cycle arrest in SKCM cell lines. ( A ) The overexpression of HOPX in A375 cells was confirmed by RT‒qPCR and Western blotting ( B ). ( C ) A CCK-8 assay was used to detect the proliferation of A375 cells. ( D ) A wound healing assay was used to detect the migration of A375 cells. ( E - H ) Transwell assays were used to detect the migration/invasion of A375 cells. ( I - J ) The apoptosis and cell cycle ( K - L ) of A375 cells were detected by flow cytometry

Article Snippet: In addition, the FastKing One Step RT‒qPCR Kit (SYBR) (TIANGEN, China) and an Eppendorf Quantitative PCR Instrument (Eppendorf, China) were used for all RT-qPCRs.

Techniques: Migration, Over Expression, Western Blot, CCK-8 Assay, Wound Healing Assay, Flow Cytometry

Journal: Biology

Article Title: Impact of Arsenic Stress on the Antioxidant System and Photosystem of Arthrospira platensis

doi: 10.3390/biology13121049

Figure Lengend Snippet: Oxidative phosphorylation pathway under As 3+ stress. ( a ) Oxidative phosphorylation pathway. ( Red indicates upregulation, while blue indicates downregulation.) ( b ) qPCR results of the key genes of the oxidative phosphorylation. (** indicate significant differences ( p < 0.05))

Article Snippet: The SYBR Green ProTaq HS Premix qPCR Kit (Accutar Biotechnology, Shanghai, China) was used for amplification, with the following program: 95 °C for 30 s, followed by 40 cycles of 95 °Cfor 5 s and 60 °C for 34 s. After the cycles, the program included 95 °C for 15 s, 65 °C for 0.05 s, and 95 °C for 0.5 s. The RT-qPCR procedure employed the two-step method, and the differential expression levels were calculated using the 2 −ΔΔCT algorithm.

Techniques: Phospho-proteomics

Journal: Biology

Article Title: Impact of Arsenic Stress on the Antioxidant System and Photosystem of Arthrospira platensis

doi: 10.3390/biology13121049

Figure Lengend Snippet: Phaeophytin a metabolism pathway under As 3+ stress. ( a ) Phaeophytin a metabolism pathway. (the arrow indicates upregulation) ( b ) qPCR results of the key genes of the photosynthetic system. (* indicate significant differences ( p < 0.05))

Article Snippet: The SYBR Green ProTaq HS Premix qPCR Kit (Accutar Biotechnology, Shanghai, China) was used for amplification, with the following program: 95 °C for 30 s, followed by 40 cycles of 95 °Cfor 5 s and 60 °C for 34 s. After the cycles, the program included 95 °C for 15 s, 65 °C for 0.05 s, and 95 °C for 0.5 s. The RT-qPCR procedure employed the two-step method, and the differential expression levels were calculated using the 2 −ΔΔCT algorithm.

Techniques:

Journal: Biology

Article Title: Impact of Arsenic Stress on the Antioxidant System and Photosystem of Arthrospira platensis

doi: 10.3390/biology13121049

Figure Lengend Snippet: qPCR results. ( a ) qPCR result of the gene. ( b ) qPCR result of MgtC family protein gene. (* indicate significant differences ( p < 0.05))

Article Snippet: The SYBR Green ProTaq HS Premix qPCR Kit (Accutar Biotechnology, Shanghai, China) was used for amplification, with the following program: 95 °C for 30 s, followed by 40 cycles of 95 °Cfor 5 s and 60 °C for 34 s. After the cycles, the program included 95 °C for 15 s, 65 °C for 0.05 s, and 95 °C for 0.5 s. The RT-qPCR procedure employed the two-step method, and the differential expression levels were calculated using the 2 −ΔΔCT algorithm.

Techniques: